Analysis Note

ControlColcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde

Application

Detect phospho-Ser/Thr-Pro MPM-2 also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IHC, IP & WB.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.

Immunogen

Mitotic human HeLa cell cytosolic lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Purified

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.

Format: Purified

Quality

routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells

Specificity

Recognizes phosphorylated serines or threonines when followed by a proline.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

varies depending on the protein detected